At present, relevant enterprises usually use a one-step method to prepare molybdenum-vanadium-aluminum-iron quaternary intermediate alloys, which have a low production cost and simple operation. After secondary vacuum refining, the gas, impurities and inclusions in the alloy can be further removed, the element distribution can be improved, the number of crystallization defects and segregation can be reduced, and the alloy quality can be improved. However, since aluminium and iron do not easily undergo direct thermal reactions via thermodynamics , the primary molybdenum- vanadium-aluminium alloy was first prepared via the thermite method. An appropriate amount of Fe was subsequently added during the second vacuum induction refining process to obtain the molybdenum-vanadium-aluminum-iron quaternary intermediate alloy. By testing the elemental composition and analysing the phase composition, microstructural morphology, and properties, new preparation processes for high-quality molybdenum-vanadium-aluminum-iron quaternary intermediate alloy were explored. The results show that the content of O and N in the alloy decreases significantly and that the surface quality of the alloy is good after vacuum induction refinement. The Mo30V30Fe5Al35 alloy is mainly composed of V0.5Mo0.5,Al5Mo, Al86Fe14 and Fe2Mo3 phases, and the structure is relatively uniform. The average density and hardness of the obtained alloys are 6.2 g/cm3 and 68.86 HRC, respectively.